I think he is finished with you and your continued insecurity. Prior to him going on a business trip with a colleague were you always questioning his fidelity? It sounds like he's pretty fed up with always being accused of not being trustworthy.
I think you're with the wrong man if you are that insecure and you feel like you do that he does nothing to reassure you. I did notice some deflecting rather then answering direct questions on his part, though.
Anyway, you're handling your insecurity in every way wrong. You shouldn't be bringing this up when he's contacting you from his trip. You sound like a jealous psycho. Instead of doing what you're doing, you should be keeping yourself and your mind busy with friends and your own interests and activities and just enjoying your conversation/facetime with him when he has the time to connect with you.
You are showing a bad side of yourself to him. If you're that uneasy about his trip with her then you should discuss your reaction to it when he gets back and if you can't resolve your anxiety then you should leave him.
“The willingness to accept responsibility for one’s own life is the source from which self-respect springs.” ~Joan Didion